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insta gene matrix dna preparation kit  (Bio-Rad)


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    Structured Review

    Bio-Rad insta gene matrix dna preparation kit
    Insta Gene Matrix Dna Preparation Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1159 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/insta+gene+matrix+dna+preparation+kit/InstaGene+Matrix/10__5458_slash_jag__jag__JAG___2013_008-27-100-105
    Average 96 stars, based on 1159 article reviews
    insta gene matrix dna preparation kit - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Characterization of Mannose Isomerase from a Cellulolytic Actinobacteria Thermobifida fusca MBL10003
    Article Snippet: .. First, a 453 bp gene fragment was obtained by PCR using forward and reverse primer designed from N-terminal amino acid sequences of the enzyme purified with sequential five step (DEAE-Sepharose, Resource ISO, Resource PHE, DEAECOSMOgel and Superdex 200HR, Pharmacia AB., Uppsala, Sweden) column chromatography, and also its end-proteinase Glu-C digested fragment.21) Then, unknown 5′- and 3′- regions were amplified by three step thermal asymmetric interlaced PCR (TAIL-PCR)22,23) using combination of three sets of forward and reverse long specific primers, and three short arbitrary degenerate primers designed from the gene fragment sequence above, MBL10003 genomic DNA as template prepared with an Insta-Gene Matrix DNA preparation kit (Bio-Rad Laboratories Inc., Hercules, USA), TaKaRa LA Taq Polymerase, and GC buffer (Takara Bio Inc., Tokyo, Japan). .. Finally, PCR was performed using forward (5′-CCTCCACGCGGCGCCGTGCGCGTATGCCGTTC-3′) and reverse (5 ′-CCATTCCCGGCCTGCCCCGGCCGGGCGCGCGG-3′) primer designed according to the sequence of TAIL-PCR fragment, genomic DNA as template, TaKaRa LA Taq Polymerase, and LA PCR buffer (Takara Bio Inc.).

    Purification:

    Article Title: Characterization of Mannose Isomerase from a Cellulolytic Actinobacteria Thermobifida fusca MBL10003
    Article Snippet: .. First, a 453 bp gene fragment was obtained by PCR using forward and reverse primer designed from N-terminal amino acid sequences of the enzyme purified with sequential five step (DEAE-Sepharose, Resource ISO, Resource PHE, DEAECOSMOgel and Superdex 200HR, Pharmacia AB., Uppsala, Sweden) column chromatography, and also its end-proteinase Glu-C digested fragment.21) Then, unknown 5′- and 3′- regions were amplified by three step thermal asymmetric interlaced PCR (TAIL-PCR)22,23) using combination of three sets of forward and reverse long specific primers, and three short arbitrary degenerate primers designed from the gene fragment sequence above, MBL10003 genomic DNA as template prepared with an Insta-Gene Matrix DNA preparation kit (Bio-Rad Laboratories Inc., Hercules, USA), TaKaRa LA Taq Polymerase, and GC buffer (Takara Bio Inc., Tokyo, Japan). .. Finally, PCR was performed using forward (5′-CCTCCACGCGGCGCCGTGCGCGTATGCCGTTC-3′) and reverse (5 ′-CCATTCCCGGCCTGCCCCGGCCGGGCGCGCGG-3′) primer designed according to the sequence of TAIL-PCR fragment, genomic DNA as template, TaKaRa LA Taq Polymerase, and LA PCR buffer (Takara Bio Inc.).

    Column Chromatography:

    Article Title: Characterization of Mannose Isomerase from a Cellulolytic Actinobacteria Thermobifida fusca MBL10003
    Article Snippet: .. First, a 453 bp gene fragment was obtained by PCR using forward and reverse primer designed from N-terminal amino acid sequences of the enzyme purified with sequential five step (DEAE-Sepharose, Resource ISO, Resource PHE, DEAECOSMOgel and Superdex 200HR, Pharmacia AB., Uppsala, Sweden) column chromatography, and also its end-proteinase Glu-C digested fragment.21) Then, unknown 5′- and 3′- regions were amplified by three step thermal asymmetric interlaced PCR (TAIL-PCR)22,23) using combination of three sets of forward and reverse long specific primers, and three short arbitrary degenerate primers designed from the gene fragment sequence above, MBL10003 genomic DNA as template prepared with an Insta-Gene Matrix DNA preparation kit (Bio-Rad Laboratories Inc., Hercules, USA), TaKaRa LA Taq Polymerase, and GC buffer (Takara Bio Inc., Tokyo, Japan). .. Finally, PCR was performed using forward (5′-CCTCCACGCGGCGCCGTGCGCGTATGCCGTTC-3′) and reverse (5 ′-CCATTCCCGGCCTGCCCCGGCCGGGCGCGCGG-3′) primer designed according to the sequence of TAIL-PCR fragment, genomic DNA as template, TaKaRa LA Taq Polymerase, and LA PCR buffer (Takara Bio Inc.).

    Amplification:

    Article Title: Characterization of Mannose Isomerase from a Cellulolytic Actinobacteria Thermobifida fusca MBL10003
    Article Snippet: .. First, a 453 bp gene fragment was obtained by PCR using forward and reverse primer designed from N-terminal amino acid sequences of the enzyme purified with sequential five step (DEAE-Sepharose, Resource ISO, Resource PHE, DEAECOSMOgel and Superdex 200HR, Pharmacia AB., Uppsala, Sweden) column chromatography, and also its end-proteinase Glu-C digested fragment.21) Then, unknown 5′- and 3′- regions were amplified by three step thermal asymmetric interlaced PCR (TAIL-PCR)22,23) using combination of three sets of forward and reverse long specific primers, and three short arbitrary degenerate primers designed from the gene fragment sequence above, MBL10003 genomic DNA as template prepared with an Insta-Gene Matrix DNA preparation kit (Bio-Rad Laboratories Inc., Hercules, USA), TaKaRa LA Taq Polymerase, and GC buffer (Takara Bio Inc., Tokyo, Japan). .. Finally, PCR was performed using forward (5′-CCTCCACGCGGCGCCGTGCGCGTATGCCGTTC-3′) and reverse (5 ′-CCATTCCCGGCCTGCCCCGGCCGGGCGCGCGG-3′) primer designed according to the sequence of TAIL-PCR fragment, genomic DNA as template, TaKaRa LA Taq Polymerase, and LA PCR buffer (Takara Bio Inc.).

    Sequencing:

    Article Title: Characterization of Mannose Isomerase from a Cellulolytic Actinobacteria Thermobifida fusca MBL10003
    Article Snippet: .. First, a 453 bp gene fragment was obtained by PCR using forward and reverse primer designed from N-terminal amino acid sequences of the enzyme purified with sequential five step (DEAE-Sepharose, Resource ISO, Resource PHE, DEAECOSMOgel and Superdex 200HR, Pharmacia AB., Uppsala, Sweden) column chromatography, and also its end-proteinase Glu-C digested fragment.21) Then, unknown 5′- and 3′- regions were amplified by three step thermal asymmetric interlaced PCR (TAIL-PCR)22,23) using combination of three sets of forward and reverse long specific primers, and three short arbitrary degenerate primers designed from the gene fragment sequence above, MBL10003 genomic DNA as template prepared with an Insta-Gene Matrix DNA preparation kit (Bio-Rad Laboratories Inc., Hercules, USA), TaKaRa LA Taq Polymerase, and GC buffer (Takara Bio Inc., Tokyo, Japan). .. Finally, PCR was performed using forward (5′-CCTCCACGCGGCGCCGTGCGCGTATGCCGTTC-3′) and reverse (5 ′-CCATTCCCGGCCTGCCCCGGCCGGGCGCGCGG-3′) primer designed according to the sequence of TAIL-PCR fragment, genomic DNA as template, TaKaRa LA Taq Polymerase, and LA PCR buffer (Takara Bio Inc.).



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